Our Labs

Peptide synthesis and purification

One of the coolest and most unique capabilities of our lab is our focus on peptide synthesis.  Once peptide or peptoid sequences of interest are identified, we can put our hypotheses to the test by creating our own, highly pure ligands and libraries.  We are experienced with a wide variety of matrices, protecting groups (and subsequent deprotection protocols), and peptide chemistries.

  • Biotage Alstra Initiator Automated Peptide Synthesizer
  • Biotage Syro I Automated Peptide Synthesizer
  • Biotage Isolera Prime Accelerated Chromatographic Isolation
  • Fume hood has been setup to perform 4 simultaneous deprotection or drying procedures
Chromatographic equipment

In order to carry out separations research on our specific and pseudo specific ligands, we do small scale testing on HPLC columns.   We also perform analytical HPLC on small molecules, calibrating them so that we can calculate drug release profiles and concentrations of valuable product.

  • 3 Waters Alliance 2690/2690D Separations Modules
  • 2 Waters 626 Pumps with 2487 UV Detectors
Cell and viral culture

Are you interested in separating from a specific culture, but don’t have a working ligand?  Many of our processes can work in a wide variety of complex solutions.  Typical CHO s solutions are acquired from the BTEC facility here at NC State, but we have the capability to perform lab scale cultures in house.  We have also acquired cell culture fluid from some of our wonderful collaborators.

Our lab also has a history of viral production and PEG precipitation.   We have also performed phage display in the past, in addition to growing bacterial cultures for separations purposes.

Analytical

Our lab analyzes data on a regular basis to confirm our hypotheses and create new ones.

  • Biotek Synergy H1 Multi-Mode Reader
  • Biotek µQuant Monochromatic Microplate Spectrophometer
  • Millipore Milli-Q™ Integral System
  • Savant Refrigerated Vapor Trap
  • Edwards Freeze Dryer Modulyo
  • Eppendorf Centrifuge 5810 R and 5417 R

The list of analytical equipment will be updated soon!